Transformation of Host cells - The Veterinary Site

Transformation of Host cells


Transformation of host cells

(by MAAZ AHMED SIDDIQUI)

       Transformation
       Inserting genes into plasmids.
       The piece of DNA or gene of interest is cut from its original DNA source using a restriction enzyme and then pasted into the plasmid by ligation.
       The plasmid containing the foreign DNA is now ready to be inserted into bacteria. This process is called transformation.
       COMPETENCE 
              is the ability of a cell to alter its genetics by taking up extracellular ("naked") DNA from its environment in the process called transformation .
Selection and screening in plasmid transformation
         -a mixture of relatively few transformed cells and non-transformed cells, a  
          method is necessary to select for the cells that have acquired the plasmid.
       The plasmid therefore requires a selectable marker such that those cells without the plasmid may be killed or have their growth arrested. 
       Antibiotic resistance is the most commonly used marker for prokaryotes.
       The transforming plasmid contains a gene that confers resistance to an antibiotic that the bacteria are otherwise sensitive to. The mixture of treated cells is cultured on media that contain the antibiotic so that only transformed cells are able to grow.


Transformation of Host cells Transformation of Host cells Reviewed by Maaz ahmed siddiqui on 12 May Rating: 5

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